Invitrogen™ eIF3d Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA527411

Product Code. 13431337

Description

Recommended positive controls: HeLaS3, mouse liver, PC-12, Rat2. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (92%), Xenopus laevis (91%), Pig (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

eIF3D is a protein belonging to the largest of the eukaryotic translation initiation factor family, eukaryotic translation initiation factor-3 (eIF3). eIF3 is a multiprotein complex composed of at least 13 different subunits. eIF3D binds to the 40S ribosomal subunit and thus helps in maintaining the 40S and 60S ribosomal subunits in a dissociated state. An important role of eIF3D is the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and promotes mRNA binding. eIF3D is the major RNA binding subunit of the eIF3 complex. It associates with the subunit p170 of eIF-3. Ubiquitous expression of eIF3D is seen in most of the tissues and it is phosphorylated upon DNA damage, likely by ATM or ATR.

Specifications

• Antigen: eIF3d
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EIF3D
• Gene Alias: 66/67kDa; AA407891; actinin alpha 4; ACTININ-4; Actn4; alpha actinin 4; alpha-actinin-4; eIF3 p66; EIF3D; eIF3d {ECO:0000255; eIF3p66; eIF3-p66; Eif3s7; eIF-3-zeta; eIF3-zeta; eIF-3-zeta {ECO:0000255; eukaryotic translation initiation factor 3 subunit 7; eukaryotic translation initiation factor 3 subunit 7 {ECO:0000255; Eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3 subunit D {ECO:0000255; eukaryotic translation initiation factor 3, subunit 7 (zeta); eukaryotic translation initiation factor 3, subunit 7 zeta; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; eukaryotic translation initiation factor 3, subunit D; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; FSGS; FSGS1; HAMAP-Rule:MF_03003}; non-muscle alpha-actinin 4; RP5-1119A7.12-003; si:dkey-165I8.6; translation initiation factor eIF3 p66; translation initiation factor eIF3 p66 subunit; wu:fb12a03; zgc:64097
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 362952, 55944, 8664
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.63 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O15371, O70194, Q6AYK8
• Gene Symbols: EIF3D
• Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF3D. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG