Invitrogen™ EGF Polyclonal Antibody

Sheep Polyclonal Antibody

Brand:  Invitrogen™ PA546935

Product Code. 15597422

Description

In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse EGF is observed and less than 0.05% cross-reactivity with recombinant human EGF is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Specifications

• Antigen: EGF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EGF
• Gene Alias: AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
• Host Species: Sheep
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25313
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P07522
• Gene Symbols: EGF
• Immunogen: E. coli-derived recombinant rat EGF Asn974-Arg1026.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG