Invitrogen™ EBP50 Monoclonal Antibody (EBP-10)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA119291

Product Code. 11524592

Description

This antibody reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein. For use in Western blot applications, MA1-19291 should be placed in a vertical incubator for 60 minutes. A suggested positive control for this product is RAJI human lymphoma cell lysate. Western Blot: Reducing conditions.

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

Specifications

• Antigen: EBP50
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: O14745
• Gene Symbols: SLC9A3R1
• Immunogen: Bacterially produced, recombinant, full-length human NHERF1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: EBP-10
• Conjugate: Unconjugated
• Gene: SLC9A3R1
• Gene Alias: EBP50; EBP-50; ERM-binding phosphoprotein; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; Nherf; NHE-RF; Nherf1; NHERF-1; NPHLOP2; Regulatory cofactor of Na(+)/H(+) exchanger; SLC9A3 regulator 1; Slc9a3r1; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 9368
• Content And Storage: 4°C, do not freeze
• Form: Liquid