Invitrogen™ E2F5 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA585578

Product Code. 16322515

Description

Keep as concentrated solution. Predicted reactivity: Mouse (91%). Positive Control: A549, H1299, HCT116, mouse placenta, HA-human E2F5-transfected 293T, mouse leukocyte treated with CFA. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.

Specifications

• Antigen: E2F5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: E2F5
• Gene Alias: AU024671; E2F transcription factor 5; E2F transcription factor 5, p130-binding; E2f5; E2F-5; Transcription factor E2F5
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13559, 1875
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q15329, Q61502
• Gene Symbols: E2F5
• Immunogen: Recombinant protein encompassing a sequence within the center region of human E2F5. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG