Invitrogen™ E. coli (strain K12) H-NS Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5117621

Product Code. 17200124

Description

This antibody has been tested in direct-ELISA.

A DNA-binding protein implicated in transcriptional repression (silencing) (PubMed:333393, PubMed:2128918, PubMed:8890170, PubMed:8913298, PubMed:9398522, PubMed:16963779, PubMed:17046956, PubMed:23543115). Also involved in bacterial chromosome organization and compaction (PubMed:6379600, PubMed:10982869, PubMed:21903814). H-NS binds tightly to AT-rich dsDNA and inhibits transcription (PubMed:2512122, PubMed:16963779, PubMed:17435766, PubMed:17881364, PubMed:23543115). Binds upstream and downstream of initiating RNA polymerase, trapping it in a loop and preventing transcription (PubMed:11714691). Binds to hundreds of sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779, PubMed:17046956, PubMed:23543115). Many of these loci were horizontally transferred (HTG); this offers the selective advantage of silencing foreign DNA while keeping it in the genome in case of need (PubMed:17046956, PubMed:17881364, PubMed:26789284). Suppresses transcription at many intragenic sites as well as transcription of spurious, non-coding RNAs genome-wide (PubMed:24449106). Repression of HTG by H-NS is thought to allow their DNA to evolve faster than non-H-NS-bound regions, and facilitates integration of HTG into transcriptional regulatory networks (PubMed:26789284). A subset of H-NSStpA-regulated genes also require Hha (andor Cnu, ydgT) for repression; Hha and Cnu increase the number of genes DNA bound by H-NSStpA and may also modulate the oligomerization of the H-NSStpA-complex (PubMed:23543115). The protein forms 2 clusters in the nucleoid which gather hns-bound loci together, bridging non-contiguous DNA, and causes DNA substantial condensation (PubMed:21903814). Binds DNA better at low temperatures than at 37 degrees Celsius; AT-rich sites nucleate H-NS binding, further DNA-binding is cooperative and this cooperativity decreases with rising temperature (PubMed:17435766, PubMed:17881364). Transcriptional repression can be inhibited by dominant-negative mutants of StpA or itself (PubMed:8755860). May effect transcriptional elongation (PubMed:25638302). Can increase translational efficiency of mRNA with suboptimal Shine-Dalgarno sequences (PubMed:20595230). Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD (PubMed:17010156). Plays a role in flagellar function (PubMed:11031114). Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its repression is antagonized by LeuO (PubMed:20132443, PubMed:20659289). Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150 (PubMed:7934818). Overexpression suppresses secY24, a temperature-sensitive mutation (PubMed:1537791). Has also been reported to activate transcription of some genes (PubMed:4566454, PubMed:338303, PubMed:2128918).

Specifications

• Antigen: E. coli (strain K12) H-NS
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: hns
• Gene Alias: DNA-binding protein H-NS; Heat-stable nucleoid-structuring protein
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 58460405, 75203350
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid

• Applications: Western Blot
• Concentration: 2.615 mg/mL
• Formulation: PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
• Gene Accession No.: P0ACF8
• Gene Symbols: hns
• Immunogen: Recombinant Escherichia coli DNA-binding protein H-NS protein (39-137aa).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG