Invitrogen™ DUT Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA141084

Product Code. 11598061

Description

Suggested positive control: antigen standard for DUT (transient overexpression lysate), Hela whole cell extract, HeLa.

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Specifications

• Antigen: DUT
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Dut
• Gene Alias: 5031412I06Rik; 5133400F09Rik; D2Bwg0749e; Deoxyuridine 5'-triphosphate nucleotidohydrolase; deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; deoxyuridine triphosphatase; Dut; Dutp; dUTP diphosphatase; dUTP nucleotidohydrolase; dUTP pyrophosphatase; dUTPase; FLJ20622; PIP4; PPAR-interacting protein 4
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 1854
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.05% BSA and 0.02% sodium azide
• Gene Accession No.: P33316
• Gene Symbols: Dut
• Immunogen: KLH-conjugated synthetic peptides corresponding to amino acids 25-44 and 113-130 of human dUTPase.
• Quantity: 200 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG