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dsRNA Antibody Pack, Novus Biologicals™

Product Code. 18362682 Shop All Bio Techne Products
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3 Vials
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Pack of 4
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18362682 3 Vials Pack of 4
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Product Code. 18362682 Supplier Novus Biologicals Supplier No. NBP311403

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Description

Includes

This pack contains 1 vial each of: NBP3-11395 (100 ug); NBP3-11396 (100 ug); NBP3-11397 (100 ug)

Mouse Monoclonal Antibody

dsRNA Monoclonal specifically detects dsRNA in Multi-Species samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Dot Blot, Immunoaffinity Purification.
TRUSTED_SUSTAINABILITY

Specifications

Antigen dsRNA
Applications ELISA, Immunohistochemistry, Immunofluorescence, Dot Blot, Affinity Purification
Classification Monoclonal
Conjugate Unconjugated
Dilution ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Dot Blot, Immunoaffinity Purification
Formulation After reconstitution the mAbs will be in PBS without any stabilisers or preservatives at a concentration of 1 mg/ml
Gene Alias Double-stranded RNA
Host Species Mouse
Immunogen Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clones.
Purification Method Protein A purified
Quantity 3 Vials
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Mouse monoclonal antibodies NBP3-11395, NBP3-11396, and NBP3-11397 recognize double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognized by all three antibodies, although in some assays the affinity of NBP3-11395 and NBP3-11396 to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens. NBP3-11397 shows higher affinity to poly(I).poly(C) than to the other dsRNA antigens, although the difference of apparent binding constants may vary under different experimental conditions.
Reconstitution The lyophilised samples should each be reconstituted with 100 ul sterile distilled water.
Target Species Multi-species
Content And Storage Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
Form Purified
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