Invitrogen™ DREF Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA527525

Product Code. 13401637

Description

Recommended positive controls: 293T, A431, Raji. Predicted reactivity: Rhesus Monkey (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.

Specifications

• Antigen: DREF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ZBED1
• Gene Alias: Ac-like transposable element; ALTE; BED-type zinc finger domain-containing protein 1; DNA replication-related element binding factor; DREF; dREF homolog; hDREF; KIAA0785; Putative Ac-like transposable element; TRAMP; ZBED1; Zinc finger BED domain-containing protein 1; zinc finger BED-type containing 1; zinc finger, BED-type containing 1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9189
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 40% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O96006
• Gene Symbols: ZBED1
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human DREF. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG