Thermo Scientific™ DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1702

DPNI 10U/UL 2500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10304240

Quantity Price
1 £ 115.0 / 2500 units
EU Stock 52
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Description and Specification

Specification

Product Name DpnI
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 2,500U

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA.