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Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, Invitrogen™

Donkey Polyclonal Secondary Antibody

Brand:  Invitrogen A21206

296.65 GBP valid until 2024-06-30
Use promo code "21742" to get your promotional price.



Code : 50

Additional Details : Weight : 0.00454kg

Product Code. 10424752

  • £331.00 / Each
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications

Specifications

Rabbit IgG (H+L) Highly Cross-Adsorbed
Polyclonal
Alexa Fluor 488
Donkey
purified
RUO
Rabbit
Liquid
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
2 mg/mL
PBS with 5mM sodium azide; pH 7.5
Gamma Immunoglobins Heavy and Light chains
1 mg
Secondary
4° C, store in dark
IgG
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