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STEMCELL Technologies DNase I

Product Code. 15964601
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25 mg
100 mg
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15964601 25 mg 25mg
17139281 100 mg 100mg
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Product Code. 15964601 Supplier STEMCELL Technologies Supplier No. 07469

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Description

For digestion of DNA

  • Deoxyribonuclease I (DNase I) is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds
  • DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage
  • DNase I preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5'-phosphate and 3'-hydroxyl groups (Bernardi et al.) DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.)

Specifications

For Use With (Application) Digestion of DNA
Quantity 25 mg
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