Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, HEPES

Product Code.	Quantity	unitSize
11584456	10 x 500 mL	5000mL
11574456	500 mL	500mL

Product Code. 11584456

Brand: Gibco™ 32430100

Description

DMEM, high glucose, GlutaMAX™ Supplement, HEPES

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. Gibco™ DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ DMEM product made in our Grand Island facility (10564-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Concentration: 1 X
• Form: Liquid
• Product Type: Dulbecco's Modified Eagle Medium
• With Additives: High Glucose, GlutaMAX, Phenol Red, HEPES, Phenol red
• Without Additives: No Sodium Pyruvate
• For Use With (Application): Mammalian cell culture
• Quantity: 10 x 500 mL
• Certifications/Compliance: ISO 13485 standards, FDA
• pH: 7.1 to 7.3
• Content And Storage: 2 to 8°C
• Description: Dulbecco's Modified Eagle Medium (DMEM), 10 x 500mL, Liquid, 1 X Concentration, 7.1 to 7.3 pH, With High Glucose, HEPES, GlutaMAX™, Phenol Red, 12 Months Shelf Life, 270 to 310mOsm/kg Osmolality, For Mammalian Cell Culture