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DL-Dithiothreitol, MP Biomedicals™
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Quantity:
5 g
Description
- Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes.
- Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
- DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
- DTT is less pungent and is less toxic than 2-mercaptoethanol.
- DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH).
- In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution.
Specifications
Melting Point | 40°C to 50°C |
Color | White |
pH | 4.0 to 6.0 (0.1 M in H2O, 25°C) |
Flash Point | 113°C / 235.4°F (lit.) |
Quantity | 5 g |
UN Number | UN3335 |
Beilstein | 1719757 |
Solubility Information | Soluble in water (50mg/mL - clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether. |
Grade | ≥99.5% |
Physical Form | Powder |
Safety and Handling
Product Identifier
- DL-Dithiothreitol, electrophoresis grade
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P321-Specific treatment (see on this label).
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : DL-Dithiothreitol
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