Thermo Scientific Chemicals DL-1,4-Dithiothreitol, 99%, for biochemistry

Codice prodotto.	Quantity	unitSize
15274654	500 g	500g
10448730	1 g	1g
10458730	5 g	5g
10215550	25 g	25g
10063023	100 g	100g

Codice prodotto. 15274654 Fornitore Thermo Scientific Chemicals N. del fornitore 165685000

Descrizione

1,4-Dithiothreitol, CAS # 3483-12-3, is a reducing agent commonly used to reduce disulphide bonds in protein studies, such as western blot SDS-page electrophoresis. | CAS: 3483-12-03 | C₄H₁₀O₂S₂ | 154.25 g/mol

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General description

• 1,4-Dithiothreitol (DTT or Cleland's reagent) reduces disulfide bonds in proteins, leading to loss of native conformation
• DTT is not stable in solution and should be prepared in small aliquots prior to use; the use of EDTA in the solution can increase the half-life of DTT
• Typically used at a final concentration of 0.1M for protein stabilization; recommended to create a 1M stock

Applications

• Protects thiol groups from oxidation and acts as a chemical chelator in solution; maintains proteins in the denatured state
• Common applications include SDS-page electrophoresis and protein isolation

Specifica

Identificatori chimici

• CAS: 12-3-3483
• MDL Number: MFCD00004877
• InChI Key: VHJLVAABSRFDPM-IMJSIDKUSA-N
• Synonym: dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt
• PubChem CID: 446094
• ChEBI: CHEBI:42170
• IUPAC Name: (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
• SMILES: C(C(C(CS)O)O)S

• Absorbance: (1M soln. in water), (1 M soln. in water) at 0nm, 0.035 max. at 500nm
• Melting Point: 39.0°C to 44.0°C
• Color: White
• Boiling Point: 125.0°C to 130.0°C (2.0 mmHg)
• Flash Point: >110°C
• Infrared Spectrum: Authentic
• Assay Percent Range: 98.5% min. (Iodimetry)
• Molecular Formula: C₄H₁₀O₂S₂
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• Quantity: 500 g
• Beilstein: 01, III, 2360
• Merck Index: 15, 3419
• Solubility Information: Solubility in water: freely soluble. Other solubilities: freely soluble in ethanol,acetone,ether,,chloroform and ethyl acetate
• Transmittance: 99% min. (c=5, 660nm, water) 1cm cell
• Molecular Weight (g/mol): 154.25
• Formula Weight: 154.25
• Percent Purity: 99%
• Grade: Biochemistry
• Physical Form: Powder
• Chemical Name or Material: DL-1, 4-Dithiothreitol

Sicurezza e movimentazione

Identificativo prodotto

• DL-1,4-Dithiothreitol

Avvertenza

• Warning

Categoria di pericolo

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Indicazioni di pericolo

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H302-Harmful if swallowed.

Consigli di prudenza

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

SDS

RUO – Research Use Only