Invitrogen™ DKK1 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHDKK1X5

Product Code. 17175419

Description

The Human DKK1 ELISA quantitates Hu DKK1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DKK1. Principle of the method The Human DKK1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Specifications

• Accession Number: O94907
• Assay Sensitivity: 100 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 22943
• Interassay CV: <12%
• Kit Contents: Wash Buffer, Stop Solution, Standard, Biotinylated Detection Antibody, Adhesive Plate Covers, Chromogen, Pre-coated 96 well plates, SAV-HRP, Assay Diluent Concentrate
• Quantity: 5 x 96 Tests
• Research Discipline: Signal Transduction
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 122.9 pg/mL to 30,000 pg/mL
• Calibration Range: 100 pg/mL to 30,000 pg/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: DKK-1,SK
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L
• Synonym: UNQ492/PRO1008, DKK-1, SK
• Test Time: 1 hr. 20 min.