Invitrogen™ DIO3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5139919

Product Code. 17804440

Description

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. Immunogen sequence: IRKHFLGRRR RGQPEPEVEL NSEGEEVPPD DPPICVSDDN RLCTLASLKA VWHGQKLDFF Highest antigen sequence indentity to the following orthologs: Rat - 29%, Mouse - 90%.

DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

Specifications

• Antigen: DIO3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DIO3
• Gene Alias: 5DIII; D3; deiodinase, iodothyronine type III; deiodinase, iodothyronine, type 3; deiodinase, iodothyronine, type III; Dio3; DIOIII; I79_015795; iodothyronine deiodinase 3; iodothyronine deiodinase, placental type; Itdi3; LOW QUALITY PROTEIN: thyroxine 5-deiodinase; thyroxine 5-deiodinase; thyroxine deiodinase; thyroxine deiodinase type III (selenoprotein); TXDI3; Type 3 DI; type 3 iodothyronine selenodeiodinase; type III iodothyronine deiodinase; type-III 5' deiodinase; type-III 5'-deiodinase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1735
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 0.05 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P55073
• Gene Symbols: DIO3
• Immunogen: Recombinant protein corresponding to Human DIO3. Recombinant protein control fragment (Product #RP-109364).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG