Invitrogen™ DGKE Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA553558

Product Code. 15791491

Description

Immunogen sequence: RDTDLFSQPT YCCVCAQHIL QGAFCDCCGL RVDEGCLRKA DKRFQCKEIM LKNDTKVLDA MPHHWIRGNV PLCSYCMVCK QQCGCQPKLC DYRCIWCQKT VHDECMKNSL KNEKCDFGEF KNLIIPPSYL Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 86%.

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and - gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK- delta (130 kDa) and DGK-i (130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK- epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK- zeta (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-theta (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.

Specifications

• Antigen: DGKE
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Dgke
• Gene Alias: AHUS7; C87606; DAG kinase epsilon; DAGK5; DAGK6; DGK; DGKE; DGK-epsilon; Diacylglycerol kinase epsilon; diacylglycerol kinase, epsilon; diacylglycerol kinase, epsilon 64kDa; diglyceride kinase epsilon; EC 2.7.1.107; NPHS7
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8526
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P52429
• Gene Symbols: Dgke
• Immunogen: Recombinant protein corresponding to Human DGKE. Recombinant protein control fragment (Product #RP-90372).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG