Invitrogen™ DFFB Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA519891

Product Code. 12888102

Description

In Western blot applications, this antibody detects a band at ~40kDa. A suggested positive control is mouse kidney tissue lysate. PA5-19891 can be used with blocking peptide PEP-0019.

Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis which is a cell death process removing toxic and/or useless cells during mammalian development can be induced by a variety of stimuli, including the death factors of the tumor necrosis factor family, or UV irradiation, anticancer drugs, and survival factor deprivation. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA. Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Currently, fourteen members of the caspase family have been identified, of which seven mediate apoptosis.

Specifications

• Antigen: DFFB
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DFFB
• Gene Alias: 2410008J01Rik; 40kDa; 5730477D02Rik; AU018859; CAD; caspase-activated deoxyribonuclease; caspase-activated DNase; caspase-activated nuclease; Cpad; CPAN; DFF2; DFF-2; DFF40; DFF-40; DFFB; Didff; DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase); DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, 40 kD, beta subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta subunit; DNase inhibited by DNA fragmentation factor; RP13-531C17.1; RP23-254N4.2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13368, 84359
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: O54788, Q99N34
• Gene Symbols: DFFB
• Immunogen: A peptide corresponding to 17 amino acids near the center of murine CAD.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG