Macherey-Nagel™ Detoxification Buffer ERB (1000 mL)

Innovative detoxification buffer removes endotoxins and proteins from plasmid DNA for use in transfection. Replacement buffer solution for use with NucleoSpin™ Plasmid/96 Plasmid Transfection-grade kits.

Brand:  Macherey-Nagel™ 740495.1000

Product Code. 15867606

Description

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

• Purifies high-copy plasmids for transfection
• Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
• Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
• Ultra-fast procedure simplifies the work-flow
• High-throughput — 96-well plates allow for easy automation and supports plasmid screening
• Downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

• Color: Colorless
• Formulation: Triethanolamine; 2-Propanol; Water
• Quantity: 1000 mL
• Odor: Alcoholic
• Solubility: Soluble in Water
• Product Type: Detoxification buffer ERB

• For Use With (Equipment): NucleoSpin Plasmid/96 Plasmid Transfection-grade full and core kit
• pH: 5.5 to 6.5
• CAS: 102-71-6
• Content And Storage: Room Temperature (18°C to 25°C)
• Form: Liquid