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Macherey-Nagel™ Detoxification Buffer ERB
Detoxification Buffer ERB (1000 mL) for transfection-grade Plasmid DNA Isolation
Brand: Macherey-Nagel™ 740495.1000
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Description
Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.
- Purifies high-copy plasmids for transfection
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
- Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
- Ultra-fast procedure simplifies the work-flow
- High-throughput — 96-well plates allow for easy automation and supports plasmid screening
- Downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications
Colorless | |
Triethanolamine; 2-Propanol; Water | |
1000 mL | |
Alcoholic | |
Soluble in Water | |
Detoxification buffer ERB |
NucleoSpin Plasmid/96 Plasmid Transfection-grade full and core kit | |
5.5 to 6.5 | |
102-71-6 | |
Room Temperature (18°C to 25°C) | |
Liquid |
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