Eurofins DiscoverX DDR2 Protein, Active

Product Code.	Quantity	unitSize
17360374	10 μg	10µg
17370374	250 μg	250µg

Product Code. 17360374 Supplier Eurofins DiscoverX Supplier No. 14579

Description

Active, N-terminal His6-tagged, recombinant, human DDR2 amino acids 467-end, for use in Kinase Assays.

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein.

GenBank NM_006182. The recombinant protein contains the amino acid substitution S642A (native coordinates) with respect toGenBank NM_006182. This conflict is reported inGenBank accession n° NM_022563, BC052998, BQ678490, BU168475 and BQ682363.

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Molecular Weight (g/mol): 48 kDa
• Quantity: 10 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal 6His
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥46%
• Protein: DDR2, active