Cytokeratin, pan Antibody (AE-1/AE-3), Biotin, Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NBP2-33200B

Product Code. 18701004

Description

Cytokeratin, pan Monoclonal specifically detects Cytokeratin, pan in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile, Zebrafish samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).

Specifications

• Antigen: pan Cytokeratin
• Classification: Monoclonal
• Conjugate: Biotin
• Gene Symbols: KRT1
• Immunogen: This Cytokeratin, pan Antibody (AE-1/AE-3) was developed against total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
• Quantity: 0.1 mL
• Research Discipline: Apoptosis, Cancer, Cell Biology, Cellular Markers, Signal Transduction
• Gene ID (Entrez): 3848
• Target Species: Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Reptile, Zebrafish
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: AE-1/AE-3
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular)
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

For Research Use Only