Cytokeratin, HMW Antibody (AE-3), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Brand:  Novus Biologicals NBP2-29404-20ug

Product Code. 18424112

Description

Cytokeratin, HMW Monoclonal specifically detects Cytokeratin, HMW in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin, HMW
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Western Blot 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells, ELISA 1-5 ug/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 ug/mL, Immunohistochemistry-Paraffin 0.5-1 ug/mL, Immunohistochemistry-Frozen 0.5-1 ug/mL
• Gene Alias: CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, Keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 51350
• Target Species: Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: AE-3
• Conjugate: Unconjugated
• Gene Accession No.: Q01546
• Gene Symbols: KRT76
• Immunogen: Human epidermal keratin (Uniprot: Q01546)
• Quantity: 20 ug
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only