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Cytokeratin, HMW Antibody (AE-3), Novus Biologicals™

Product Code. 18424112 Shop All Bio Techne Products
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0.1 mg
20 ug
Unit Size:
0.10mg
20µg
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Product Code. Quantity unitSize
18424112 20 ug 20µg
18196032 0.1 mg 0.10mg
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Product Code. 18424112 Supplier Novus Biologicals Supplier No. NBP22940420ug

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Description

Mouse Monoclonal Antibody has been used in 1 publication

Cytokeratin, HMW Monoclonal specifically detects Cytokeratin, HMW in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Cytokeratin, HMW
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone AE-3
Concentration 0.2 mg/ml
Conjugate Unconjugated
Dilution Western Blot 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells, ELISA 1-5 ug/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 ug/mL, Immunohistochemistry-Paraffin 0.5-1 ug/mL, Immunohistochemistry-Frozen 0.5-1 ug/mL
Gene Accession No. Q01546
Gene Alias CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, Keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9
Gene Symbols KRT76
Host Species Mouse
Immunogen Human epidermal keratin (Uniprot: Q01546)
Purification Method Protein A or G purified
Quantity 20 ug
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 51350
Test Specificity This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Target Species Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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