Invitrogen™ Cytokeratin 8 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA594954

Product Code. 16354225

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human MCF-7 whole cell, human CACO-2 whole cell, human HepG2 whole cell, huamn Jurkat whole cell, rat RH35 whole cell, mouse HEPA1-6 whole cell. IHC: human intestinal cancer tissue. Flow: CACO-2 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Specifications

• Antigen: Cytokeratin 8
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: KRT8
• Gene Alias: AA960620; AL022697; AU019895; CARD2; cb186; CK8; CK-8; CYK8; CYKER; cytokeratin 8; cytokeratin 8 (370 AA); cytokeratin endo A; Cytokeratin-8; cytokeratin-A; DreK8; EndoA; etID33550.23; etID43142.23; etID43142.8; K2C8; K8; KERA; keratin 8; keratin 8, type II; keratin complex 2, basic, gene 8; keratin, type 2, gene 8; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8-like protein; keratin-8; KO; Krt-2.8; Krt2-8; krt8; LOW QUALITY PROTEIN: keratin, type II cytoskeletal 8; sb:cb186; type II cytokeratin A (no.8); type-II keratin Kb8; wu:fa20h05; wu:fa95h10; wu:fb96c06; zfk8; zf-K8
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16691, 25626, 3856
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.01mg sodium azide
• Gene Accession No.: P05787, P11679, Q10758
• Gene Symbols: KRT8
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Cytokeratin 8 (127-145aa QQQKTARSNMDNMFESYIN).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG