Cytokeratin 6 Monoclonal Antibody (LL020), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9788-82

Product Code. 15537686

Description

Description: The monoclonal antibody LL020 recognizes human Cytokeratin 6. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. Cytokeratin 6 is a type II (basic) keratin that associates with Cytokeratin 16 and Cytokeratin 17. Cytokeratin 6 is expressed in the epidermis, outer root sheath of hair follicles, oral mucosa, suprabasal cells of stratified epithelia, and glandular epithelia. Gene mutations result in inherited skin and hair disorders. Some reports suggest that LL020 may have some crossreactivity to Cytokeratin 5. Applications Reported: This LL020 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and ELISA. Applications Tested: This LL020 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Specifications

• Antigen: Cytokeratin 6
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P02538, P04259, P48668
• Gene Symbols: KRT6A, KRT6B, KRT6C
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 286887, 3853, 3854
• Content And Storage: 4° C
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Western Blot
• Clone: LL020
• Conjugate: Unconjugated
• Gene: KRT6C
• Gene Alias: 2310016L08Rik; 60-kDa keratin; BB144589; CK6A; CK-6A; CK6B; CK-6B; CK6C; CK-6C; CK6D; CK-6D; CK-6E; cytokeratin 6A; cytokeratin 6B; cytokeratin 6C; cytokeratin 6D; cytokeratin-6A; Cytokeratin-6B; cytokeratin-6C; Cytokeratin-6D; Cytokeratin-6E; Hom s 5; K6A; K6a keratin; K6B; K6C; K6D; K6E; kamp-keratin derived antimicrobial peptide; KDAMP; Ker2; keratin 6A; keratin 6A, , type II; keratin 6A, type II; keratin 6B; keratin 6B, type II; keratin 6C; keratin 6C, type II; keratin 6E; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6b; keratin complex 2, basic, gene 6c; keratin complex 2, gene 6a; keratin K6; keratin K6h; keratin, epidermal type II, K6A; keratin, epidermal, type II, K6B; keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 6B; keratin, type II cytoskeletal 6C; keratin-6A; Keratin-6-alpha; Keratin-6B; keratin-6-beta; keratin-6C; keratin-like 1 (a type II keratin sequence); Krt2-6; Krt2-6a; Krt2-6b; Krt2-6c; Krt6; Krt6a; Krt6b; KRT6C; KRT6D; KRT6E; K
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM κ

For Research Use Only.