Cytokeratin 5/8 Antibody (C-50) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18179518	Novus Biologicals NBP2-47824-0.1MG	0.1 mg

Description

Cytokeratin 5/8 Monoclonal specifically detects Cytokeratin 5/8 in Human, Mouse, Rat, Porcine, Bovine, Canine, Hamster, Ferret, Sheep, Chicken (Negative), Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: Cytokeratin 5/8
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rat, Pig, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative)
• Gene Alias: cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
• Gene Symbols: KRT5
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

• Clone: C-50
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 3852
• Immunogen: Cytoskeletal preparation from HeLa cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.