Invitrogen™ Cytokeratin 19 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA532463

Product Code. 13496977

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin tissue.

Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.

Specifications

• Antigen: Cytokeratin 19
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: KRT19
• Gene Alias: 40-kDa keratin intermediate filament; AI663979; C K1CS; CK 19; CK19; CK-19; cytokeratin 19; cytokeratin-19; EndoC; K19; K1CS; Ka19; keratin 19; keratin 19, type I; keratin complex 1, acidic, gene 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt-1.19; Krt1-19; Krt19; LOC100344434; MGC15366; Type I keratin Ka19
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3880
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.043 mg/mL
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: P08727
• Gene Symbols: KRT19
• Immunogen: Synthetic peptide derived from C-terminus of human Keratin 19.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG