Invitrogen™ Cytokeratin 14 Monoclonal Antibody (SP53)

Rabbit Monoclonal Antibody

Brand:  Invitrogen™ MA516370

Product Code. 13401107

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, squamous cell carcinoma or prostate tissue.

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.

Specifications

• Antigen: Cytokeratin 14
• Classification: Monoclonal
• Concentration: 1.17 mg/mL
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: P02533, Q61781, Q6IFV1
• Gene Symbols: KRT14
• Immunogen: Synthetic peptide corresponding to C-terminus of human keratin 14 protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: SP53
• Conjugate: Unconjugated
• Gene: KRT14
• Gene Alias: AI626930; CK14; CK-14; cytokeratin 14; cytokeratin-14; EBS3; EBS4; epidermolysis bullosa simplex, Dowling-Meara, Koebner; K14; Ka14; keratin 14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin complex 1, acidic, gene 14; keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 17; keratin-14; Koebner; Krt-1.14; Krt1-14; KRT14; LOC100737113; NFJ; Type I keratin Ka14
• Host Species: Rabbit
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 16664, 287701, 3861
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid