Invitrogen™ Cyclin E Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA585177

Product Code. 16312125

Description

Keep as concentrated solution. Predicted reactivity: Mouse (85%), Rat (85%), Chicken (80%), Bovine (86%). Positive Control: U87-MG, MCF-7, MDA-MB-231, HCT-116, HCT-116 (1 mM Hydroxyurea treatment for 24hr). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Specifications

• Antigen: Cyclin E
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CCNE1
• Gene Alias: AW538188; Ccne; Ccne1; CycE1; CYCLE; cyclin E; cyclin E1; cyclin Es; cyclin Et; E-S; G1/S-specific cyclin-E1; pCCNE1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 898
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P24864
• Gene Symbols: CCNE1
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Cyclin E1. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG