Cyclin D1 Mouse, Clone: SPM587, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 7 publications

Product Code	Brand	Quantity
18138992	Novus Biologicals NBP2-32840-0.02MG	0.02 mg
18062944	Novus Biologicals NBP2-32840-0.1MG	0.1 mg

Description

Cyclin D1 Monoclonal specifically detects Cyclin D1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cyclin D1
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Cycle and Replication, Core ESC Like Genes, mTOR Pathway, Stem Cell Markers, Wnt Signaling Pathway
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, BCL1D11S287E, cyclin D1, cyclin D1 (PRAD1: parathyroid adenomatosis 1), G1/S-specific cyclin D1, G1/S-specific cyclin-D1, PRAD1 oncogene, PRAD1B-cell CLL/lymphoma 1, U21B31
• Gene Symbols: CCND1
• Isotype: IgG2a κ
• Purification Method: Protein A purified
• Test Specificity: Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

• Clone: SPM587
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1 - 2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Primate
• Gene Accession No.: P24385, P24385, P25322, P39948
• Gene ID (Entrez): 595
• Immunogen: Human recombinant full length cyclin D1 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 36 kDa