Cyclin B1 Monoclonal Antibody (GNS11), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA514327

Product Code. 11365033

Description

MA5-14327 targets Cyclin B1 in WB, ICC/IF, IHC (P), and IP applications and shows reactivity with Hamster, Human, mouse, and Rat samples. This antibody is not recommended for NIH-3T3 cells in Western blot applications. The MA5-14327 immunogen is human cyclin B1 protein. This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

Specifications

• Antigen: Cyclin B1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P14635, P24860, P30277, Q08301
• Gene Symbols: Ccnb1
• Immunogen: Human cyclin B1 protein
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Hamster, Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: GNS11
• Conjugate: Unconjugated
• Gene: Ccnb1
• Gene Alias: Ccn-2; CCNB; Ccnb1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb1; Cycb1-rs1; Cycb-4; Cycb-5; cyclin B; cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; I79_013589; OTTHUMP00000221981; OTTHUMP00000223023
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100770842, 25203, 268697, 891
• Content And Storage: 4° C
• Form: Liquid