PeproTech CXCL16 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P200-100UG

Product Code. 17855243

Description

AA Sequence of recombinant protein: NEGSVTGSCY CGKRISSDSP PSVQFMNRLR KHLRAYHRCL YYTRFQLLSW SVCGGNKDPW VQELMSCLDL KECGHAYSGI VAHQKHLLP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CXCL16. Anti-Human CXCL16-specific antibody was purified by affinity chromatography employing an immobilized Human CXCL16 matrix. Sandwich ELISA: To detect Human CXCL16 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CXCL16 (500-P200Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CXCL16. Western Blot: To detect Human CXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses. The chemokine domain of CXCL16 contains six cysteine residues, including the four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids. The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal mucin-like stalk sequence. The extracellular domain contains 89 amino acid residues (86 a.a. residues for the murine homolog).

Specifications

• Antigen: CXCL16
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cxcl16
• Gene Alias: 0910001K24Rik; AV290116; b2b498Clo; BB024863; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 16; CXC; Cxc chemokine ligand 16; CXC motif chemokine; C-X-C motif chemokine 16; C-X-C motif chemokine ligand 16; CXCL; Cxcl16; CXCL16v1; CXCL16v2; CXCLG16; Mutant line 498; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; Srpsox; SR-PSOX; SR-PSOX/CXCL16; Transmembrane chemokine CXCL16; UNQ2759/PRO6714; zinc finger, MYND-type containing 15; Zmynd15
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 58191
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q9H2A7
• Gene Symbols: Cxcl16
• Immunogen: E.coli-derived Recombinant Human CXCL16.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody