PeproTech CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P315BT-500UG

Product Code. 30054587

Description

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat SDF-1alpha (CXCL12). Anti-Rat SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Rat SDF-1alpha by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat SDF-1alpha (500-P315) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat SDF-1alpha. Western Blot: To detect Rat SDF-1alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Rat SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P315BT-1MG will be provided as 2 x 500 μg

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: CXCL12 alpha (SDF-1 alpha)
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: CXCL12
• Gene Symbols: CXCL12
• Immunogen: E.coli-derived, 7.9 kDa Recombinant Rat SDF-1alpha.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Alias: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXC; CXC motif chemokine; C-X-C motif chemokine 12; C-X-C motif chemokine ligand 12; CXCL; Cxcl12; H-SDF-1-alpha; Intercrine reduced in hepatomas; IRH; Pbsf; pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SCYB12; SDF 1 a; SDF 1 alpha; SDF 1α; SDF1; SDF-1; SDF1 alpha; SDF-1 gamma; SDF1A; SDF-1-alpha(3-67); SDF1B; SDF-1-beta(3-72); SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; stromal cell-derived factor-1 gamma; thymic lymphoma cell-stimulating factor; TLSF; Tpar1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 24772
• Content And Storage: -20°C
• Form: Lyophilized