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CXCL10 (IP-10), PerCP-eFluor™ 710, clone: 4NY8UN, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9744-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15599426

  • £81.72 / 25 tests


Estimated Shipment date
from Supplier 26-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This 4NY8UN monoclonal antibody reacts with human CXCL10. CXCL10, also known as Interferon Inducible Protein-10 (IP-10), is a member of the CXC family of chemokines. It is secreted by monocytes, epithelial cells, and endothelial cells in response to IFN gamma or other pro-inflammatory cytokines and stimuli. CXCL10 signaling is mediated by the G protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. In addition to its chemotactic functions, CXCL10 is a potent inhibitor of angiogenesis and tumor growth and modulates expression of adhesion molecules. Elevated levels of CXCL10 are observed in response to allergic inflammation, various infections, and Alzheimer's disease.Applications Reported: This 4NY8UN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 4NY8UN antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CXCL10 (IP-10)
4NY8UN
PerCP-eFluor 710
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interferon Inducible Protein-10, CXC Motif Chemokine 10
IgG2b
RUO
Primary
Human
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