Gibco™ CTS™ TrueCut™ Cas9 Protein

Gibco™ CTS TrueCut Cas9 Protein is our highest performing CRISPR-Cas9 protein, engineered to deliver maximum ribonucleoprotein (RNP)-editing efficiency.

Product Code	Brand	Quantity
17747187	Gibco™ A45220	2.5 mg
17757187	Gibco™ A45221	5 mg

Description

Gibco CTS TrueCut Cas9 Protein is our highest performing CRISPR-Cas9 protein, engineered to deliver maximum ribonucleoprotein (RNP)-editing efficiency. This protein is manufactured in compliance with standards for Ancillary Materials for Cell, Gene and Tissue-Based Products and is subjected to stringent quality specifications. It is provided in a high-concentration and large-scale format for process development and clinical research applications.

Features of CTS TrueCut Cas9 Protein include:
• Consistent high editing efficiency—superior CRISPR-Cas9 editing efficiency in all tested cell lines including primary immune cells (>90% editing in primary T cells)
• Higher stringency manufacturing—manufactured to meet the standards for Ancillary Materials for Cell, Gene, and Tissue-Based Products including USP <1043>, Ph.Eur. 5.2.12, and ISO 20399, following the principles of 21 CFR Part 820 in a FDA-registered manufacturing site
• Aseptic manufacturing—entire process completed using single-use technology
• Extensive safety testing—includes sterility, endotoxin, mycoplasma, residual nucleic acids, and proteins
• Sterile filling—finished product is vialed in a Class A/B (Class 100 and ISO 5 equivalents) cleanroom
• Traceability documentation—COA, COO, Drug Master Files, and Regulatory Support Files are available

CTS TrueCut Cas9 Protein is a Streptococcus pyogenes Cas9 (wt) protein, produced by recombinant DNA technology in E. coli for genome editing with CRISPR technology. Cas9 protein forms a very stable RNP complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of NLS aids delivery to the nucleus, increasing the editing efficiency and on-target cleavage.

If you would like to evaluate our TrueCut Cas9 protein or conduct small-scale research experiments, we recommend using our TrueCut Cas9 Protein v2 (Cat. No. A36497).

Specifications

• Concentration: 10 mg/mL
• Product Type: Cas9 Protein
• Content And Storage: Store at –20°C.
• Expression System: E. coli
• Research Category: Genome Editing

• For Use With (Application): Cell and Gene Therapy Research, Development, and Manufacturing
• Sterility: Sterile-filtered
• Shipping Condition: Dry Ice
• Form: Liquid
• Manufacturing Quality: ISO13485

For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research. Caution: Not intended for direct administration into humans or animals.