Gibco™ CTS™ OpTmizer™ T Cell Expansion SFM, bag format

Product Code.	Quantity	unitSize
11692099	1 Bag	Each

Product Code. 11692099 Supplier Gibco™ Supplier No. A1048503

Description

CTS™ OpTmizer™ T Cell Expansion SFM, bag format

Gibco™ OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free and xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bag) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

Maintains similar phenotype and function as T-cells cultured in conventional serum supplemented medium

Consistent performance in supporting T-cell expansion

Supports high density cultures in static and WAVE Bioreactor™ cultures

Be ready for clinical research with this cGmP manufactured formula and support documentation

Versatile T Cell Culture Media
OpTmizer™ CTS™ T-Cell Expansion SFM is designed to give results equivalent to conventional serum-supplemented media. However, if desired, 2% heat-inactivated human serum (Cat. No. 34005-100) may be added to the medium to enhance viability and expansion. The use of serum, and the amount required, should be determined empirically depending on the specific T-cell culture application.

Ready for T Cell Expansion
OpTmizer™ CTS™ T-Cell Expansion SFM is designed for the expansion of human T-cells permitting CD3+ densities of >3x10⁶ cells/mL in static conditions and >2x10⁷ cells/mL in WAVE bags. Results may vary depending on the T-cell subset (e.g., Ag-specific cells). Conditions for specific T-cell culture applications should be determined empirically.

Stability of Complete Media
Complete OpTmizer™ CTS™ T-Cell Expansion SFM (basal, supplement, and L-glutamine) is stable for 4 weeks when stored in the dark at 2–8°C.

Simplify Your Transition into Clinical Research
The Gibco™ Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco™ CTS™ products deliver:

Reduced burden in qualifying reagents

Appropriate certificates of analyses and origin included

Convenient access to our Drug Master File (DMF)

Specifications

• Cell Type: T Cells
• Classification: Chemically-defined, Serum-free, Xeno-free
• Form: Liquid
• Product Type: Cell Expansion Serum Free Medium (SFM)
• Serum Level: Serum-free
• Sterility: Sterile
• With Additives: HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
• Without Additives: No Glutamine
• Quantity: 1 Bag
• Endotoxin Level: Very Low
• Included Antibiotics: No antibiotics
• Inorganic Salts: Calcium, Magnesium
• Purity or Quality Grade: Research Grade
• Content And Storage: OpTmizer™ T-Cell Expansion Basal Medium (1 x L media bag): Store at 2–8°C. Protect from light.
  
  OpTmizer™ T-Cell Expansion Supplement (1 x 26 mL): Store in the dark at 2–8°C.
• Culture Environment: CO₂
• Culture Type: Suspension Cell Culture

Frequently Asked Questions (FAQs)

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Do I need to order additional components with the CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503)?

The CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) kit comes with OpTmizer T Cell Expansion Basal Medium and OpTmizer T Cell Expansion Supplement, which are mixed together prior to use. Depending on your protocol you may need to purchase T cell growth factors such as IL-2 (Cat. No. PHC0023) and L-glutamine (Cat. No. 25030) to form a complete medium. To boost growth of human T cells, human AB serum can be added. If desired, antibiotics such as Gentamicin (Cat. No. 15750), can also be added.

Will CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) work with all types of T cells?

CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has been demonstrated to be effective for the ex vivo expansion of human CD3 T cells, including polyclonal T cell populations, antigen-specific T cells, tumor-infiltrating lymphocytes (TIL), cord blood T cells, regulatory T cells, T cells from HIV-infected donors, gene-modified T cells (both lenti- and MLV-based vectors), including cells of both the CD4+ and CD8+ phenotype.

Can CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) be used for animal T cell cultures other than human?

CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has only been tested on human T cells.

Is there a difference in formulation of the CTS OpTmizer T Cell Expansion SFM between the bottle and bag packaging formats?

The bottle and bag packaging formats of the medium have the same formulation.

Can I use CTS OpTmizer T Cell Expansion SFM for culture and expansion of mouse T cells? If not, which medium would you recommend?

We have not tested this medium on mouse T cells. If you're looking for a serum-free medium to culture mouse T cells, you can try AIM V Medium (Cat. No. 12055083 or 12055091). For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).

Can GlutaMAX be used instead of Glutamine to supplement CTS OpTmizer T-Cell Expansion SFM?

GlutaMAX cannot replace Glutamine because cells may not initially use GlutaMAX so that T cells cannot grow. We recommend adding 4 mM GlutaMAX and 2 mM Glutamine (as stated in the manual) into the CTS OpTmizer T-Cell Expansion SFM if GlutaMAX is needed.

What is the shelf life of CTS OpTmizer T-Cell Expansion SFM?

The complete CTS OpTmizer T-Cell Expansion SFM is stable for 4 weeks when stored in the dark at 2-8 degrees C. However, the two individual medium components are stable for 18 months when stored in the dark at 2-8 degrees C.