Invitrogen™ Creatine Kinase MT Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA521504

Product Code. 13248898

Description

Recommended positive controls: 293T, H1299, HeLaS3, HepG2. Predicted reactivity: Rat (98%), Xenopus laevis (88%), Rabbit (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

Specifications

• Antigen: Creatine Kinase MT
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CKMT1A
• Gene Alias: acidic-type mitochondrial creatine kinase; CKMT; CKMT1; CKMT1A; CKMT1B; Creatine kinase U-type, mitochondrial; creatine kinase, mitochondrial 1 (ubiquitous); creatine kinase, mitochondrial 1, ubiquitous; creatine kinase, mitochondrial 1A; creatine kinase, mitochondrial 1B; mia-CK; mi-CK; Mt-CK; ScCKmit; UbCKmit; ubiquitous mitochondrial creatine kinase; UMTCK; U-MtCK
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1159, 548596
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P12532
• Gene Symbols: CKMT1A, CKMT1B
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Creatine kinase MT. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG