Invitrogen™ Creatine Kinase BB Monoclonal Antibody (CK-CYK/21E10)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA533339

Product Code. 17175233

Description

Product is supplied at 1 mL (100 μL/mL). Application notes: Western blot - Under reducing conditions, antibody detected a protein with a band size of 43-46 kDa. IHC-F- tissue was fixed for 2 hours in 2% paraformaldehyde and 0.1 % glutaraldehyde in 0.1 M PB. The tissue was stored until use in 1 % paraformaldehyde. The tissue was blocked with 1% normal swine serum. IHC-P - antigen retrieval: 0.5 % pepsin in 0.01 N HCl for 15 min at 37 °C; endogenous peroxidise blocking by using 3 % H2O2 in PBS for 30 min; blocking with 1% normal swine serum for 30 min.; Purkinje cells serve as positive control and connective tissue as negative control. Positive control: Brain lysate, Human Purknije cells; Negative control: Human fibroblasts.

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Specifications

• Antigen: Creatine Kinase BB
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.1% BSA and 0.02% sodium azide
• Gene Accession No.: P00567, P07335, P12277, Q04447
• Gene Symbols: CKB
• Immunogen: Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain-type (SNSHNALKLRFPAEDEF).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: CK-CYK/21E10
• Conjugate: Unconjugated
• Gene: CKB
• Gene Alias: Bck; B-CK; brain creatine kinase; Ck3; Ck-3; CKB; Ckbb; Ckbr; CPK-B; creatine kinase B; creatine kinase B chain; creatine kinase brain; creatine kinase brain-type; Creatine kinase B-type; creatine kinase, brain; creatine kinase-B; Creatine phosphokinase B-type; Creatine phosphokinase M-type; epididymis luminal protein 211; epididymis secretory protein Li 29; HEL-211; HEL-S-29
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100009085, 1152, 12709, 24264
• Content And Storage: 4°C
• Form: Liquid