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Corning™ BioCoat™ Poly-L-Ornithine/Laminin Multiwell Plate

Enhancement of neuronal cell attachment to plastic and glass

Brand:  Corning™ 354659

Code : 67

Additional Details : Weight : 0.55000kg

Product Code. 10264611

  • £227.41 / Pack of 5
Estimated Shipment: 24-07-2024
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Description

Description

For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone. Corning BioCoat PDL/LM and PLO/LM Cultureware is suitable for culturing many different types of Peripheral Nervous System (PNS) and Central Nervous System (CNS) networks and is useful for promoting neural cell attachment and differentiation. Corning BioCoat LM/HFN Cultureware provides an in vitro environment that promotes cell attachment and extensive process formation.

Applications include:

  • Enhancement of neuronal cell attachment to plastic and glass
  • Promotion of neurite outgrowth
  • Culture of glial cells as a feeder layer for neurons
  • Construction of neural cell model systems to study CNS function, development, and diseases

Source

  • PDL, synthetic (MW 75-150 kD)
  • PLO, synthetic (MW 30-70 kD)
  • Laminin, EHS mouse tumor
  • Fibronectin, human plasma
  • NOTE: Source material tested for hepatitis B antigen and HIV-1 antibody.
  • Quality Control

    • PDL/LM and PLO/LM tested for ability to initiate differentiation (neurite outgrowth) of NG-108 rat glioma/mouse neuroblastoma cells
    • LM/HFN tested for receptor agonist induced changes in intracellular calcium using Fluo-3A (a long wavelength fluorescent calcium indicator) in primary rat cortical neuron enriched cultures
    • Tested and found negative for bacteria and fungi

    Storage

  • 2° to 8°C. Do not freeze.
Specifications

Specifications

4 x 6
Yes
Flat
2 cm2
Rectangular
Laminin/Poly-L-Ornithine
24
Lid
1/Pk.
Poly-L-Ornithine and Laminin
Clear
Polystyrene
Clear
Polystyrene
Non-sterile
Multi-well Cell Culture Plate
Enhancement of neuronal cell attachment to plastic and glass, promotion of neurite outgrowth, culture of glial cells as a feeder layer for neurons, construction of neural cell model systems to study CNS function, development and diseases
5/Cs.
5/Cs.
2°C to 8°C
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