Invitrogen™ Connexin 46 Recombinant Rabbit Monoclonal Antibody (11H16L33)

Rabbit Recombinant Monoclonal Antibody

Brand:  Invitrogen™ 700384

Product Code. 15386964

Description

This antibody is predicted to react with rat and hamster based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Connexin 46 (Cx46), also known as Gap Junction Protein Alpha-3 (GJA3), CAE3, CX46 and C2P3, maps to human chromosome 13q11-q12 and encodes a 46 kDa protein. Cx46, along with Cx50, is principally expressed in the lens of the eye. Cx46 mediates intercellular interactions during development and is necessary for the survival of neural crest cells. Cx46 forms gap junctions that connect lens fiber cells and are crucial for maintaining lens transparency and normal lens function. Mutations of Cx46 result in severe cataracts of the lens. Individual knockouts of Cx46 and Cx50 lead to changes in the rate of lens fiber cell differentiation and cell size, thus the interaction of Cx46 and Cx50 is required for proper organization of fiber cells.

Specifications

• Antigen: Connexin 46
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: Q64448
• Gene Symbols: GJA3
• Immunogen: A peptide corresponding to amino acids 131-144 of Q64448.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 11H16L33
• Conjugate: Unconjugated
• Gene: GJA3
• Gene Alias: alpha 3 connexin; Cnx46; connexin 46; connexin-46; CTRCT14; Cx43; CX46; Cxn-46; CZP3; gap junction alpha 3; gap junction alpha-3 protein; gap junction membrane channel protein alpha 3; gap junction protein alpha 3; gap junction protein, alpha 3; gap junction protein, alpha 3, 46kDa; Gja3; Gja-3
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 14611
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid