Connexin 43, Biotin, clone: Connexin43 (1A), eBioscience™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15567336	Affymetrix eBioscience 13-4759-80	25μg
15577336	Affymetrix eBioscience 13-4759-82	100μg

Description

Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.

Description: The Connexin43 monoclonal antibody recognizes mouse Connexin 43. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 43 is expressed in many tissues, including the central nervous system, heart, and bone. Connexin 43 is the most abundant connexin found in the myocardium and is also the primary connexin expressed in astrocytes. Mutations in connexin 43 are associated with ocluodentodigital dysplasia, a rare developmental disorder characterized by craniofacial and limb abnormalities. Connexin 43 may also be a promising therapeutic target in the treatment of malignant melanomas and mammary gland tumors.The monoclonal antibody Connexin43 also recognizes human and rat connexin 43.Applications Reported: This Connexin43 antibody has been reported for use in immunocytochemistry, and immunohistochemical staining of frozen tissue sections.Applications Tested: This Connexin43 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Connexin 43
• Target Species: Human, Murine, Rat
• Isotype: IgG1, kappa, kappa
• Monoclonal or Polyclonal: Monoclonal
• Format: Liquid
• Type: Antibody
• Storage Requirements: Store at 2-8°C. Do not freeze.

• Clone: Connexin43 (1A)
• Conjugate: Biotin
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

For Research Use Only.