Cytiva Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Brand:  Cytiva 17-0440-01

Product Code. 11524905

Description

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

• Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
• Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars
• Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
• Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
• Average particle size: 90μm
• Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl₂, 1mM MgCl₂, 1mM MnCl₂, and 20% ethanol

Specifications

• Binding Capacity: 30mg/mL
• Matrix: 4% Agarose
• pH: 4 to 9
• Content And Storage: 2°C to 8°C
• Ligand: Concanavalin A

• Ligand Concentration: 13mg Con A/mL Drained Medium
• Particle Size: 90 μm
• Quantity: 100 mL
• For Use With (Application): For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes

Safety and Handling

Product Identifier

• Con A Sepharose 4B, 100 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol