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Cytiva Con A Sepharose™ 4B Medium
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Quantity:
5 mL
100 mL
Unit Size:
100mL
5mL
Description
Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.
- Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
- Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Group-specific adsorbent for molecules containing sugars
- Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
- Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
- Average particle size: 90μm
- Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol
Specifications
Specifications
| Binding Capacity | 30mg/mL |
| Ligand Concentration | 13mg Con A/mL Drained Medium |
| Matrix | 4% Agarose |
| Particle Size | 90 μm |
| pH | 4 to 9 |
| Quantity | 100 mL |
| Content And Storage | 2°C to 8°C |
| For Use With (Application) | For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes |
| Ligand | Concanavalin A |
Safety and Handling
Product Identifier
- Con A Sepharose 4B, 100 ml
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : ethanol
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