Collagen II Antibody, Biotin, Novus Biologicals™

Rabbit Polyclonal Antibody

Brand:  Novus Biologicals NBP2-21663

Product Code. 18119531

Description

Collagen II Polyclonal specifically detects Collagen II in Human, Mouse, Rat, Bovine, Sheep samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Collagen II
• Classification: Polyclonal
• Conjugate: Biotin
• Formulation: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Sodium Azide
• Gene Alias: Alpha-1 type II collagen, ANFH, AOM, cartilage collagen, chondrocalcin, COL11A3, Collagen 2, collagen alpha-1(II) chain, collagen II, alpha-1 polypeptide, collagen type 2, collagen, type II, alpha 1, collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital), Collagen-2, MGC131516, SEDC
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen II and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Target Species: Human, Mouse, Rat, Bovine, Sheep, Canine, Silkworm
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Concentration: LYOPH
• Dilution: Western Blot 1:5000-1:10000, ELISA 1:10000-1:50000, Immunohistochemistry 1:200-1:1000, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:200-1:1000
• Gene Accession No.: P02458
• Gene Symbols: COL2A1
• Immunogen: Collagen II from human knee cartilage and bovine nasal cartilage. (Uniprot: P02458)
• Quantity: 0.1 mg
• Research Discipline: Cytoskeleton Markers, Extracellular Matrix, Stem Cell Markers
• Gene ID (Entrez): 1280
• Reconstitution: Reconstitute with 100 ul deionized water (or equivalent)
• Content And Storage: Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.

For Research Use Only