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STEMCELL Technologies ClonaCell™-CHO CD Medium

Product Code. 17121036
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90 mL
Unit Size:
90mL
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17121036 90 mL 90mL
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Product Code. 17121036 Supplier STEMCELL Technologies Supplier No. 03815

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Description

Chemically defined, animal component-free, serum-free, protein-free, glutamine-free semi-solid methylcellulose-based medium for selection and cloning of CHO cells

  • ClonaCell™-CHO CD Medium is a methylcellulose-based semi-solid medium recommended for selection and cloning of Chinese hamster ovary (CHO) cells
  • This medium is chemically defined, protein-free, and animal component-free
  • It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthetase (GS) selection systems
  • Benefits of semi-solid cloning: Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies
  • This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening
  • Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning
  • Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion

Specifications

Cell Line CHO
For Use With (Application) Cell Culture; Semi-Solid Cloning
Quantity 90 mL
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