Eurofins DiscoverX CHK2 (R145W) Protein, Active

Product Code. 17320644

Brand: Eurofins DiscoverX 14740

Description

Active, N-Terminal GST and C-Terminal 6His-tagged, recombinant human CHK2, amino acid residues 5-end, containing the R145W mutation, for use in Kinase Assays.

Checkpoint kinase (CHK) is activated by a broad spectrum of DNA-damaging agents, including DNA-strand-breaking agents such as ionizing radiation and topoisomerase inhibitors, and those agents that cause replication stress such as ultraviolet light, hydroxyurea and 5-fluorouracil. CHK2 might have a redundant and supportive role in checkpoints, but its role in ionizing radiation-induced apoptosis is more prominent. However, its role in other DNA-damage-induced apoptosis mechanisms is less well established. CHK2 inhibitors are expected to sensitize proliferating cells to chemotherapy-induced apoptosis.

GenBank NP_009125

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Molecular Weight (g/mol): 89.6 kDa
• Quantity: 10 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal GST and C-terminal 6His tags
• Expression System: Recombinant enzyme expressed in E. coli cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥50%
• Protein: CHK2 (R145W), active