Invitrogen™ Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488

Product Code.	Quantity	unitSize
10749174	1 mg	Each

Product Code. 10749174

Brand: Invitrogen™ A21441

Description

Chicken Polyclonal Secondary Antibody

To minimize cross-reactivity, these chicken anti-rabbit IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against human and mouse IgG prior to conjugation. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without sig...

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG (H+L) Cross-Adsorbed
• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 2 mg/mL
• Conjugate: Alexa Fluor 488
• Formulation: PBS with 5mM sodium azide; pH 7.5
• Host Species: Chicken
• Immunogen: Gamma Immunoglobins Heavy and Light chains.
• Purification Method: Purified
• Quantity: 1 mg
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Rabbit
• Content And Storage: 4°C, store in dark
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgY