Thermo Scientific™ Cfr42I (SacII)

The Cfr42I (SacII) restriction enzyme recognizes CCGC^GG sites and cuts best at 37°C in B buffer (Isoschizomers: KspI, SacII, Sfr303I, SgrBI, SstII).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0202

CFR42I (SACII) 10U/UL 5X1200U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.02000kg

Product Code. 10369679

Quantity Price
1 £105.00 / Pack of 5
EU Stock 2
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Description and Specification


Product Name Cfr42I (SacII)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 5 x 1,200U


  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities


  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP


Cfr42I activity is affected by high salt concentration. Trace amounts of sodium chloride remaining in the substrate DNA after completion of upstream applications may inhibit enzyme activity and result in impaired DNA cleavage. Certain sites in lambda DNA are difficult to cleave with Cfr42I, the same as with its prototype SacII. At least two copies of Cfr42I recognition site are required for efficient cleavage.