missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10175594

Thermo Scientific™ CellSensor™ TrkC-NFAT-bla CHO-K1 Cell Line

Product Code. 10175594
Change view
Click to view available options
Quantity:
1 Vial
Unit Size:
Each
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10175594 1 Vial Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10175594 Supplier Thermo Scientific™ Supplier No. K1515

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

Neurotrophins (NGF, BDNF, NT-3, and NT-4) and their transmembrane receptors (TrkA, TrkB, TrkC, and P75NTR) play important roles in the regulation of neuronal and non-neuronal cell proliferation, differentiation, survival, and death.

Neurotrophins (NGF, BDNF, NT-3, and NT-4) and their transmembrane receptors (TrkA, TrkB, TrkC, and P75NTR) play important roles in the regulation of neuronal and non-neuronal cell proliferation, differentiation, survival, and death. Neurotrophin signaling also mediates higher-order neuronal activities, such as learning, memory, and behavior. Alterations in neurotrophin levels and their receptors have been implicated in neurodegenerative disorders such as Alzheimer's disease and Huntington's disease, as well as psychiatric disorders. Neurotrophins propagate their signal through activating multiple signaling pathways. One of the signaling pathways of NT-3 (the ligand for TrkC) activates phospholipase C, releasing DAG and IP3, increasing downstream intracellular calcium, and activating protein kinase C. This in turn promotes the translocation of the transcription factor-nuclear factor of activated T-cells (NFAT)-from the cytosol into the nucleus, resulting in NFAT-dependent transcription.

The CellSensor™ TrkC-NFAT-bla CHO-K1 Cell Line was engineered by integrating the human TrkC expression plasmid into the genome of the CellSensor™ NFAT-bla CHO-K1 cell line, which is engineered to express beta-lactamase under the control of NFAT. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, and in cryopreserved cells, and has been validated for Z'-factor and EC50 values under optimized conditions using NT-3. Additional testing information using various small-molecule inhibitors and RNAi has been performed.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ TrkC-NFAT-bla CHO-K1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Description CellSensor™ TrkC-NFAT-bla CHO-K1 Cell Line, 1Vial, ∽ 8,000,000 cells/vial at 8,000,000 cells/mL Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, Signaling Pathway Druggable Target, Adherent Growth Properties, Liquid Nitrogen Storage
Detection Method Fluorescence
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, kinase biology, kinase pathway analysis, pathway biology, cellular pathway analysis assays, cellSensor™ cellular pathway assays
Product Line CellSensor
Quantity 1 Vial
Sample Type CHO-K1
Target Entry PKC and calcium pathway, TRKC
Type CHO-K1 Cells
Cell Line CHO-K1
System Type CellSensor™
Druggable Target Signaling Pathway
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.