Invitrogen™ CEA Monoclonal Antibody (3G12)

Product Code.	Quantity	unitSize
12827822	100 μL	100µL

Product Code. 12827822 Supplier Invitrogen™ Supplier No. MA515799

Description

Mouse Monoclonal Antibody

MA5-15799 targets CEA in indirect ELISA, IF and WB applications and shows reactivity with Human samples. The MA5-15799 immunogen is purified recombinant fragment of human CEA expressed in E. Coli. MA5-15799 detects CEA which has a predicted molecular weight of approximately 77kDa.

CEA (Carcino Embryonic Antigen, CD66e) is synthesized during development in the fetal gut, and re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA is a member of a large family of glycoproteins, a useful tumor marker for adenocarcinoma, and found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. CEA was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, CEA was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.

Specifications

• Antigen: CEA
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 3G12
• Concentration: Not determined
• Conjugate: Unconjugated
• Formulation: Ascites with 0.03% sodium azide
• Gene: Ceacam5
• Gene Accession No.: P06731
• Gene Alias: 1600029H12Rik; Carcinoembryonic antigen; carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; CEACAM5; Meconium antigen 100; OTTHUMP00000199033; pregnancy-specific glycoprotein 30; Psg30; sCD66e; soluble CD66e
• Gene Symbols: Ceacam5
• Host Species: Mouse
• Immunogen: Purified recombinant fragment of human CEA expressed in E. Coli.
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1048
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1